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Ribobio co
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Lentigen Inc
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Merck KGaA
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VectorBuilder GmbH
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ACGT Inc
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GeneBay Inc
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SunBio Inc
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Image Search Results
Journal: International Journal of Molecular Medicine
Article Title: MicroRNA-449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice
doi: 10.3892/ijmm.2020.4502
Figure Lengend Snippet: Identification of the 3′-UTR seed region of SCN2B mRNA targeted by miR-449a. A dual-luciferase activity assay for SCN2B 3′UTR-wt or SCN2B 3′UTR-mut detected firefly and luciferase activities following transfection with (A) miR-449a, (B) miR-9, (C) miR-34a-5p mimic or miR-NC. (A): Relative luciferase activity of miR-449a mimics co-transfected with SCN2B 3′UTR-wt or 3′UTR-mut (left panel) and seed regions of SCN2B gene directly regulated by miR-449a (right panel). NC, negative control; UTR, untranslated region; wt, wild-type; mut, mutant; SCN2B, sodium channel voltage-gated beta 2.
Article Snippet: The artificially cloned sequences were inserted downstream of the
Techniques: Luciferase, Activity Assay, Transfection, Negative Control, Mutagenesis